Objective: The present study examined the relationship between quality of work life and performance of Railway Headquarter employees in Hormozgan province. Method: The research method is descriptive-correlation and 150 employees working in Railway Headquarter in Hormozgan were chosen through stratified random sampling. In this study, two questionnaires including a 16-item quality of work life questionnaire (Walton) and a 14-item questionnaire (Achiu performance) were used to collect the data. After collecting the data, analysis was done by using descriptive statistics and inferential statistics such as Spearman, Kendall and multiple regressions. Results: The results showed a direct and significant relationship between the quality of work life and human resource performance. Besides, there is a significant relationship between the quality of work life (fair and adequate payment, safe workplace, developing human capabilities, providing opportunities for growth and continuous security, social integration, constitutionalism in the organization, social dependence) and the employees' performance.